Canine Parvovirus (CPV), belonging to the genus Parvovirus within the family Parvoviridae, is responsible for causing severe and highly contagious infectious diseases in dogs. Clinically, it manifests in two primary forms: hemorrhagic enteritis and myocarditis. Both forms are characterized by high mortality rates, strong infectivity, and rapid disease progression, with particularly elevated infection and mortality rates observed in puppies. Therefore, reliable and effective detection plays a critical role in guiding prevention, diagnosis, and treatment.
The nitrocellulose membrane is pre-marked with a test line (T) and a control line (C). The T line is coated with antibody a, which specifically binds to CPV antigens.The conjugate pad contains fluorescent nanomaterial-labeled antibody b, another antibody designed to specifically recognize CPV.During testing:CPV in the sample binds to the nanomaterial-labeled antibody b, forming a complex.The complex migrates upward through the chromatographic membrane and binds to antibody a on the T line, creating a sandwich structure.When exposed to excitation light, the nanomaterial emits a fluorescent signal.The signal intensity is directly proportional to the CPV concentration in the sample.
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