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Canine Pancreatic Lipase Test Kit (cPL)
Detection Purpose
Canine pancreatic lipase (cPL) is an enzyme produced by the pancreas in dogs. Pancreatitis, an inflammatory condition of the pancreas, is generally divided into acute pancreatitis and chronic pancreatitis. Acute pancreatitis is characterized by neutrophil infiltration, pancreatic necrosis, peripancreatic fat necrosis, edema, and tissue injury. Chronic pancreatitis is characterized by pancreatic fibrosis and atrophy. Compared with acute pancreatitis, chronic pancreatitis is usually less acutely harmful but occurs more frequently. When a dog has pancreatitis, the pancreas is damaged, leading to a rapid increase in the levels of canine pancreatic lipase in the blood. Currently, pancreatic lipase is one of the best specific indicators for the diagnosis of canine pancreatitis.
Detection Principle
This product utilizes fluorescence immunochromatography to quantitatively detect cPL content in whole blood and serum or plasma. The basic principle is as follows: T and C lines are respectively marked on the nitrocellulose membrane. The T line is coated with an antibody a that specifically recognizes the cPL antigen. The binding pad is sprayed with another fluorescent nanomaterial-labeled antibody b that specifically recognizes cPL. The cPL in the sample first combines with the nanomaterial-labeled antibody b to form a complex. Then, through chromatography, the complex moves upward to binds to the T-line antibody a, forming a sandwich structure. When the test strip is irradiated by excitation light, the nanomaterial emits a fluorescent signal, and the intensity of this signal is positively correlated with the concentration of cPL in the sample.
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