Canine/Feline Cholyglycine Test Kit (CG)

Detection Purpose 

Cholyglycine (CG) is one of the conjugated bile acids formed by the combination of cholic acid and glycine. Cholyglycine is the core bile acid component of serum in late trimester of pregnancy. When hepatocytes are damaged, their ability to intake CG decreases.When bile stagnation occurs, the liver is unable to excrete cholic acid, leading to an increase in the level of CG flowing back to the bloodstream, thus increasing the blood CG level .

Detection Principle 
This product utilizes fluorescence immunochromatography to quantitatively detect the CG content in dog/cat blood. The basic principle is as follows: T and C lines are respectively marked on the nitrocellulose membrane. The T line is coated with an antigen a that specifically binds to the antibody. The binding pad is sprayed with another fluorescent nano-material-labeled antibody b that specifically recognizes antigen a. The antigen in the sample first combines with the nano-material-labeled antibody b to form a complex. Then, through chromatography, the complex moves upward. The more antigen in the sample that binds to the complex, the less fluorescent antibody binds to the T line. When the test strip is irradiated by excitation light, the nanomaterial emits a fluorescent signal , and the intensity of this signal is negatively correlated with the concentration of antigen in the sample.