Feline coronavirus infection is very common in cats. It is generally believed that the virus can cause diarrhea and infectious peritonitis symptoms.The body would produce feline coronavirus antibody when the cat is infected by coronavirus.
New-Test Biotech’s previous studies have shown that the antibody level in the serum and ascites of sick cats with typical symptoms of feline infectious peritonitis was much higher than that of sick cats with intestinal infection caused by common coronavirus. When the sick cats with suspected symptoms of infectious peritontis has a high level of antibody detected in the blood or ascites, it will indicate that the cats have a high possibility of suffering from infectious peritonitis. In addition, antibody detection has a certain significance in ruling out negative diagnosis. The possibility of infectious peritonitis can be ruled out if the antibody in the blood is very low and the antibody does not significantly increase after 7 days of interval monitoring.
Clinical Significance:
1) Quantitative monitoring of coronavirus antibody concentration to determine whether the cat is being infected by coronavirus (non carrying);
2) A high concentration of antibody being detected indicates an increased possibility of being infected with infectious peritonitis.
3) Making a negative elimination diagnosis for infectious peritonitis.
FCoV IgG antibody in cat blood was quantitatively detected by fluorescence immunochromatography. Basic principle: There are T and C lines on the nitrate fiber membrane respectively. The binding pad is sprayed with a fluorescent nanomaterial marker that can specifically recognize FCoV IgG antibody. The FCoV IgG antibody in the sample first combines with the nanomaterial marker to form a complex, and then goes to the upper chromatography. The complex combines with the T-line, and when the excitation light irradiation, the nanomaterial emits fluorescence signal. The strength of the signal was positively correlated with the concentration of FCoV IgG antibody in the sample.
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