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Fish Cortisol Test Kit (fCor)
Detection Purpose
The detection of cortisol in fish is of great significance. As a stress hormone, the content of cortisol in fish will change significantly when fish are under environmental stressors, such as water quality deterioration, temperature changes, overfishing, etc. By detecting the level of cortisol in fish, we can accurately evaluate the degree of stress that fish are under and then determine their health status. This is not only helpful for the aquaculture industry to optimize the farming conditions, prevent fish diseases and improve the breeding efficiency, but also provides key scientific basis for the protection of wild fish resources and the maintenance of the balance of the ecosystem.
Detection Principle
This product is a fluorescent immunochromatographic assay for the quantitative analysis of fCortisol in serum/plasma.
Basic principle: T and C lines are drawn on the nitrocellulose membrane. The T line is encapsulated with an antigen a that specifically recognizes Cortisol antibody, and another nanomaterial-conjugated antibody b that specifically recognizes Cortisol is sprayed on the binding pad. When the sample is applied, Cortisol in the sample first binds to the nanomaterial-conjugated antibody b to form a complex. During the chromatography process, this complex moves upward along the membrane and competes with the antigen a on the T line. Since the complex already has Cortisol bound to antibody b, it cannot bind to the antigen a on the T line, and thus the complex passes through the T line without being captured. On the contrary, when there is no Cortisol in the sample, the antibody b can bind to the antigen a on the T line. The nanomaterial emits a fluorescent signal when it is irradiated by the excitation light, and the strength of the signal is inversely proportional to the concentration of Cortisol in the sample.
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