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Canine N-terminal Pro-Brain Natriuretic Peptide Test Kit (cNT-proBNP)
Purpose of Detection
Canine N-terminal pro-B-type natriuretic peptide (cNT-proBNP): It is a marker of cardiac load in dogs. Its levels increase in cases such as increased cardiac load after exercise, valvular heart disease, and heart failure.
Reference Recommendations for NEW-TEST Canine NT-proBNP: When a dog has valvular disease (MMVD), a concentration of 800-2500 nmol/L suggests that the dog's MMVD may be in the b1-b2 stage. Most dogs may show no obvious symptoms. A concentration of 2500-4500 nmol/L (b2-C stage) indicates that the dog has a high risk of developing heart failure (HF) within 6-12 months. It is recommended to perform further echocardiogram examinations or annual physical examinations. When the concentration is > 4500 nmol/L, the likelihood of heart failure is extremely high.
Detection Principle
This product utilizes fluorescence immunochromatography to quantitatively detect the cNT-proBNP content in serum or plasma. The basic principle is as follows: T and C lines are respectively marked on the nitrocellulose membrane. The T line is coated with an antibody a that specifically recognizes cNT-proBNP. The binding pad is sprayed with another fluorescent nano-material-labeled antibody b that specifically recognizes cNT-proBNP. The cNT-proBNP in the sample first combines with the nano-material-labeled antibody b to form a complex. Then, through chromatography upwards, the complex moves upwards and binds to T line antibody a, forming a sandwich structure. When the test strip is irradiated by excitation light, the nanomaterial emits a fluorescent signal, and the intensity of this signal is positively correlated with the concentration of cNT-proBNP in the sample.
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