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Feline Cardiac Biomarkers 4-in-1 Combo Test Kit (fNT-proBNP/fCTnI/fD-D/FHW Ag)
Detection Purpose
Feline N-terminal pro-brain natriuretic peptide (fNT-proBNP) is a substance secreted by myocardial cells in the feline ventricle, which can be used as a detection indicator for corresponding heart failure. The concentration of fNT-proBNP in the blood is related to the severity of the disease. Therefore, fNT-proBNP can not only assess the severity of acute and chronic heart failure, but also serve as an indicator of its prognosis status.
Troponin is a complex of three proteins, troponin T (TnT), troponin C (TnC), and troponin I (TnI), which exists exclusively in cardiac tissues and is one of the most sensitive and specific serum markers of cardiomyocyte injury.
D-dimer is the simplest fibrin degradation product, and elevated levels of D-dimer are indicative of a hypercoagulabale state and secondary hyperfibrinolysis. Therefore, the mass concentration of D-dimer is very meaningful for the diagnosis, efficacy evaluation, and prognosis of thrombotic diseases.
Feline Heartworm (FHW), a parasitic round nematode, can enter the heart and pulmonary arterial system to damage the cardiac and pulmonary blood vessels and tissues, greatly affecting the health of pets. Therefore, the reliable and effective testing plays a positive guiding role in prevention, diagnosis and treatment.
Detection Principle
This product utilizes fluorescence immunochromatography to quantitatively detect the content of fNT-proBNP, TnI, D-dimer and FHW in cat’s serum/plasma. The basic principle is as follows: T and C lines are respectively marked on the nitrocellulose membrane. The T line is coated with an antibody a that specifically recognizes fNT-proBNP (TnI, D-dimer, FHW). The binding pad is sprayed with another fluorescent nano-material-labeled antibody b that specifically recognizes fNT-proBNP (TnI, D-dimer, FHW). The fNT-proBNP (TnI, D-dimer, FHW) in the sample first combines with the nano-material-labeled antibody b to form a complex. Then, through chromatography, the complex moves upward to binds to T line antibody a, forming a sandwich structure. When the test strip is irradiated by excitation light, the nanomaterial emits a fluorescent signal, and the intensity of this signal is positively correlated with the concentration of fNT-proBNP (TnI, D-dimer, FHW) in the sample.
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